|
KMID : 0616120100410010021
|
|
Korean Journal of Pharmacognosy
2010 Volume.41 No. 1 p.21 ~ p.25
|
|
|
Inhibitory Effect of Heartwood of Rhus verniciflua Stokes on Lipid Accumulation in 3T3-L1 Cells
|
|
Kim Sei-Gun
Ryu Dong-Young
Kim Do-Kuk
Ko Da-Hyung
Kim Yun-Kyung
Lee Young-Mi
Jung Hyun-Ju
|
|
|
Abstract
|
|
|
The MeOH extract of Rhus verniciflua heartwood inhibited lipid accumulation in 3T3-L1 adipocytes. Chromatographic methods including of silica gel, RP-18 and high-pressure liquid chromatography isolated sulfuretin and fisetin from the extract as active constituents. The isolated compounds, especially sulfuretin, strongly inhibited lipid accumulation in
adipocytes. The treatment of sulfuretin and fisetin led to decreased expression of peroxisome proliferator-activated receptorgamma (PPARγ), as an important transcription factor in fat cell differentiation, which was equal to the decrease in the quercetin positive control. The presence of a hydroxyl group (C-5) in quercetin compared to fisetin, and the presence of C-2 double bonds in fisetin compared with fustin increased the inhibitory effect of lipid accumulation.
|
|
|
KEYWORD
|
|
|
Rhus verniciflua, flavonoid, sulfuetin, adipocyte, PPARγ
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|